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投稿时间:2024-09-26 修订日期:2024-10-25
投稿时间:2024-09-26 修订日期:2024-10-25
中文摘要: 针对酸性矿山废水高浓度硫酸盐和金属离子以及低pH的污染情况,从矿区煤矸石堆的底泥和浸出液中筛选鉴定出一株硫酸盐还原菌,根据其形态特征以及16SrRNA基因序列进行鉴定,研究碳源、温度、pH值、初始硫酸盐浓度对该菌株生长和还原硫酸盐的影响,并利用该菌株处理酸性矿山废水。菌株经过16SrRNA基因测序后,鉴定为Citrobacter freundii,命名为S1(NCBI登录号:PP273014)。菌株S1在35℃、pH为7.0、SO42-浓度为2000mg/L的条件下生长最为旺盛。菌株S1对酸性矿山废水中TFe、Mn2+、Zn2+和SO42-均有一定的处理效果,接种7d后去除率分别为81.96%、35.08%、52.92%和63.24%,废水pH从4.38提高到了6.6左右。
中文关键词: 硫酸盐还原菌 酸性矿山废水 重金属离子 硫酸盐还原
Abstract:A strain of sulfate-reducing bacteria was isolated from the bottom sediment and leachate of the coal gangue pile in the mining area. This strain was identified according to its morphological characteristics and 16SrRNA gene sequence. It was found to be capable of reducing sulfate and metal ions, and to be tolerant of low pH. The morphological characteristics and 16SrRNA gene sequence were employed to investigate the impact of carbon source, temperature, pH, and initial sulfate concentration on the growth and sulfate reduction of the strain. Furthermore, the strain was utilized to treat acidic mine wastewater. The strain was identified as Citrobacter freundii after 16SrRNA gene sequencing and named S1 (NCBI accession number: PP273014). Strain S1 exhibited the greatest growth rate at 35°C, pH 7.0, and a sulfate concentration of 2000 mg/L. Strain S1 demonstrated efficacy in the treatment of TFe, Mn2+, Zn2+ and SO42- in acid mine wastewater, with removal rates of 81.96%, 35.08%, 52.92% and 63.24%, respectively. Additionally, the pH of the wastewater exhibited an increase from 4.38 to approximately 6.6 following a seven-day period of inoculation.
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基金项目:国家重点研发计划课题(2019YFC1805003),酸性煤矸石堆场排水系统研究及污水立体阻隔材料与技术
作者 | 单位 | |
曹梦竺 | 徐州生物工程职业技术学院 | 2370152267@qq.com |
王铎* | 中国矿业大学 环境与测绘学院 | wangduo1194@163.com |
马婷 | 中国矿业大学 环境与测绘学院 |
引用文本:
曹梦竺,王铎,马婷.SRB的分离鉴定及其对酸性矿山废水的处理[J].有色金属(冶炼部分),2024(12):157-166.
Cao Mengzhu,Wang Duo,Ma Ting.Isolation and characterization of SRB and its treatment of acid mine wastewater[J].Nonferrous Metals (Extractive Metallurgy),2024(12):157-166.
曹梦竺,王铎,马婷.SRB的分离鉴定及其对酸性矿山废水的处理[J].有色金属(冶炼部分),2024(12):157-166.
Cao Mengzhu,Wang Duo,Ma Ting.Isolation and characterization of SRB and its treatment of acid mine wastewater[J].Nonferrous Metals (Extractive Metallurgy),2024(12):157-166.