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| Study on the Breeding of Uranium Leaching Strains and Their Physiological and Biochemical Characteristics |
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Received:November 10, 2020
Revised:November 18, 2020
Accepted:November 20, 2020
Published Online:March 24, 2021
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| DOI：doi：10.3969/j.issn.1007-7545.2021.04.011 |
| KeyWord:strain breeding; physiological and biochemical characteristics; in-situ leaching of uranium; leaching uranium strains(512SCK-Af-17) |
| Author | Institution |
| Liu Jia-chen |
东华理工大学水资源与环境工程学院 东华理工大学核资源与环境国家重点实验室 |
| Liu Jin-hui |
东华理工大学水资源与环境工程学院 东华理工大学核资源与环境国家重点实验室 |
| Xu Ling-ling |
东华理工大学水资源与环境工程学院 东华理工大学核资源与环境国家重点实验室 |
| Zhou Yi-peng |
东华理工大学水资源与环境工程学院 东华理工大学核资源与环境国家重点实验室 |
| Zhang Yi-nuo |
东华理工大学水资源与环境工程学院 东华理工大学核资源与环境国家重点实验室 |
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| Abstract: |
| In order to make full use of limited production of bacterial liquid to extract uranium in situ, it is necessary to study biological leaching technology of underground in situ bacteria. One of the key technologies is whether iron-oxidation activity of uranium-leaching strains in in-situ leaching can meet requirements of underground uranium leaching. Acidithiobacillus ferriooxidans(512SCK-AF-17), a Acidithiobacillus ferriooxidans with ferric oxidation activity, was isolated and purified from a uranium deposit in Xinjiang, and its physiological and biochemical characteristics were systematically studied at low temperature environment of 20 ℃ and in-situ leaching tail solution. The results show that after continuous culture and gradual acclimation, ferric oxidation activity of strain is significantly enhanced, and 5 g/L Fe2+ can be completely oxidized in the system at low temperature of 20 ℃ for 30 hours. The shortest growth cycle of these strains is 16 h cultured in tail liquid with Fe2+ concentration of 3 g/L, initial pH value of 1.8, and temperature of 30 ℃. Fe2+ oxidation rate is the highest in culture environment with 20% inoculations, initial pH value of 1.8, and Fe2+ concentration of 5~10 g/L. This research results provide a scientific basis for application of biological in-situ leaching technology of sandstone-type uranium deposits. |
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