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| Test Study on Immobilized Continuous Culture of Thiobacillus Ferrooxidans |
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Received:January 12, 2021
Revised:January 18, 2021
Accepted:January 22, 2021
Published Online:May 20, 2021
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| DOI：doi：10.3969/j.issn.1007-7545.2021.06.008 |
| KeyWord:Thiobacillus ferrooxidans; vaccination rate; immobilization; continuous culture; oxidation rate of ferrous iron |
| Author | Institution |
| ZHOU Yue-chen |
东华理工大学水资源与环境工程学院 |
| ZHENG Zhi-hong |
东华理工大学水资源与环境工程学院 |
| LI Si-yun |
东华理工大学水资源与环境工程学院 |
| SUN Zhan-xue |
东华理工大学水资源与环境工程学院 |
| WANG Xue-gang |
东华理工大学水资源与环境工程学院 |
| KE Ping-chao |
东华理工大学水资源与环境工程学院 |
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| Abstract: |
| Based on previous studies, a proposal is put forward to carry out immobilization culture experiment of Thiobacillus ferrooxidans by adopting 50% inoculation rate and reducing inoculation rate successively. The test results show that this method can complete immobilization of bacteria within 10 days after inoculation for 9 times, and average oxidation rate of ferrous iron can reach 0.31-0.61 g/(L?h) after complete immobilization. Under the same conditions, average oxidation rate of ferrous iron by immobilized cells is 5 times as high as that of free cells with 20% inoculation rate and 4 times as high as that with 40% inoculation rate. At continuous culture stage, ferrous oxidation rate of highly immobilized bacteria with different materials and different columns can reach 6.67 g/(L?h). The carrier column with height of 55 cm and filled with zeolite which can be selected as subsequent column leaching test to culture active bacteria solution. |
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