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| Isolation and characterization of SRB and its treatment of acid mine wastewater |
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Received:September 26, 2024
Revised:October 25, 2024
Accepted:October 28, 2024
Published Online:November 17, 2024
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| DOI： |
| KeyWord:Sulfate-reducing bacteria, Acid mine drainage, Heavy metal ions, Sulfate reduction |
| Author | Institution |
| Cao Mengzhu |
徐州生物工程职业技术学院 |
| Wang Duo |
中国矿业大学 环境与测绘学院 |
| Ma Ting |
中国矿业大学 环境与测绘学院 |
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| Abstract: |
| A strain of sulfate-reducing bacteria was isolated from the bottom sediment and leachate of the coal gangue pile in the mining area. This strain was identified according to its morphological characteristics and 16SrRNA gene sequence. It was found to be capable of reducing sulfate and metal ions, and to be tolerant of low pH. The morphological characteristics and 16SrRNA gene sequence were employed to investigate the impact of carbon source, temperature, pH, and initial sulfate concentration on the growth and sulfate reduction of the strain. Furthermore, the strain was utilized to treat acidic mine wastewater. The strain was identified as Citrobacter freundii after 16SrRNA gene sequencing and named S1 (NCBI accession number: PP273014). Strain S1 exhibited the greatest growth rate at 35°C, pH 7.0, and a sulfate concentration of 2000 mg/L. Strain S1 demonstrated efficacy in the treatment of TFe, Mn2+, Zn2+ and SO42- in acid mine wastewater, with removal rates of 81.96%, 35.08%, 52.92% and 63.24%, respectively. Additionally, the pH of the wastewater exhibited an increase from 4.38 to approximately 6.6 following a seven-day period of inoculation. |
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